Yesterday before end work was working on microbiological test on Corn Cob, and took pics with my favorite machine, which is also xuan's favorite: AUTOCLAVE! So let me take u thru the steps of doing a microbiological experiment:

The product that we are testing:


1st u must have the agar powder, for this we are using a general agar to grow all kinds of bacteria so here it is:


then, u must weigh according to the specs, for this case is 2.6g




Following, u must pour water to make it into a solution, rmb to use distill water..


Bring the solution to heat:


In the mean time, prepare the test tube filled with water, as u want a sterile water when doing ur experiment, to prevent contamination:


And also a number of pipette tips:


The solution have boiled :


The petri dishes are sterile, so u need not sterilise it:


This is the autoclave machine, it looks so alien, haha:


Add water in it, and place the items in the 2 tub in it, close it tightly:


The temperature and pressure is set:


The temperature will rise:


Keep rising:


And continue to rise:


Still rising:


It will go no higher than this, as there is a sensor, due to the pressure water boils at 121degree instead of 100 degree:


Leave it for 15 - 20 mins, at the mean time, on ur UV light at the laminar flow hood:


After that, pour ur agar on the plates allow it overnight, following day, set ur equipments in ur fumehood, make sure there is no contamination:


Do your serial dilution, and dun forget to vortex it to ensure homogeneously mixture:



After that, pipette the serial dulution onto your agar plates:




Rmb to soak ur spreader in ethanol, and set it on fire before usage, this is also to ensure sterile conditions:


Spread the liquid on the agar plates, please be gentle, or u will crack it...and dun forget to label them:


That is all for today's microbiological test, please to visit for the results! Hope you had fun in this boring lesson.

8:41 PM sHaRiLyN LiM